SB 415286

SB 415286 is a potent and selective cell permeable inhibitor of GSK-3α, with an IC₅₀ of 77.5 nM, and a K_i of 30.75 nM; SB 415286 is equally effective at inhibiting human GSK-3α and GSK-3β. IC50 & Target: IC50: 77.5 nM (GSK-3α)^[1]
Ki: 30.75 nM (GSK-3α)^[1] In Vitro: SB 415286 (SB-415286) inhibits human GSK-3α with an IC₅₀ of 77.5 nM, and a K_i of 30.75 nM. SB-415286 stimulates glycogen synthesis in the Chang human liver cell line with EC₅₀ of 2.9 μM. SB-415286 stimulates glycogen synthase activity in Chang human liver cells. SB-415286 induces transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells^[1]. SB 415286 (SB-415286, 5-44 μM) attenuates B65 cell loss mediated by 1 mM H₂O₂. SB-415286 (5-44 μM) causes a significant dose-dependent decrease in the fluorescence intensity of DCF, and attenuates B65 ROS production as mediated by 1 mM H₂O₂. SB-415286 (5-44 μM) also attenuates ROS production in CGN mediated by 1 mM H₂O₂^[2]. SB-415286 (50 µM) induces a substantial suppression of immunoprecipitated GSK3 activity by 97%^[3].