Methylisothiazolinone (hydrochloride)

Methylisothiazolinone hydrochloride is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone hydrochloride can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone hydrochloride can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone hydrochloride can cause mitochondrial damage in the endothelium of rat cerebral blood vessels^[1][2][3][4].
In Vitro:Methylisothiazolinone (0-8 μg/mL, 24 h) hydrochloride induces cell apoptosis and inflammatory response by activating matrix metalloproteinases (MMPs) in human bronchial epithelial cells^[1].
Methylisothiazolinone (0-2.5 μg/mL, 1 h) hydrochloride can cause mitochondrial damage in brain endothelial cells^[2].
In Vivo:Methylisothiazolinone (0.15 mg/kg; once; i.v.) hydrochloride can increase the susceptibility of rat cerebral vascular endothelium to endogenous pathological stimuli and lead to mitochondrial damage^[2].
Methylisothiazolinone (20 or 40 μL; handling skin (20 μL), days 1 and 8; intranasal instillation (40 μL), day 15) hydrochloride can cause sensitization in asthmatic mice when handled on the skin^[3].
Methylisothiazolinone (0.1875 mg/kg CMIT/MIT; once daily; 15 days; epidermal contact) hydrochloride promotes the development of atopic dermatitis in mice through Th2/Th17 related immune response dysregulation^[4].