1-Ethyl-(3-dimethylaminopropyl)carbodiimide (hydrochloride)

1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride is a carbodiimide reagent that can form nucleic acid and compounds with amide bonds. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride accelerates the formation reaction of esters, amides, and peptides, as a condensing and dehydrating agent, which are often used for polynucleotide synthesis, anhydroxydation, lactonization and esterification^[1][2]. In Vitro: Usage:
First dilute 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to 500 μg/mL, mix with the dye, the volume ratio of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the dye is 1:1, incubate at room temperature for about 20 minutes, then add them to the sample solution and incubate at room temperature for 1-2 hours.

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium^[1].
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl), is widely used for polyaniline-carbon nanotube preparation for a cholesterol biosensor, precolumn derivatization of aliphatic amines for HPLC, molecular beacons formation for DNA reseach, sensor preparation for calcium detection, the fluorescent determination of carboxylic acids, and solidphase microsequencing of peptides^[2].
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is an aminoaldehyde dehydrogenase (AMADH) inhibitor, while its inhibition on glutamate decarboxylase (GAD) is insignificant^[3].