Metamifop

Metamifop is a post-emergence lipid synthesis inhibitor herbicide, with an IC₅₀ of 41.1 nM against the carboxyltransferase (CT) domain of chloroplast acetyl-CoA carboxylase (ACCase) in barnyard grass (Echinochloa crus-galli). Metamifop inhibits the activity of the CT domain, thereby blocking fatty acid biosynthesis^[1]. In Vitro:Metamifop potently inhibits ACCase activity in Echinochloa crus-galli with an IC₅₀ of 41.1 nM^[1].
Metamifop (3 ns) binds to the dimer interface active site of the Echinochloa crus-galli CT domain via π-π interactions and protonation-dependent hydrogen bonds, with a unique binding region compared to other ACCase inhibitors, and undergoes a conformational shift in the binding site during MD simulations^[1]. In Vivo:Metamifop (12.5-90 g a.i. ha⁻¹; foliar spray) dose-dependently damages chloroplast ultrastructure, alters CT domain gene expression in early growth stages, and efficiently inhibits ACCase activity (IC₅₀ = 41.1 nM) in susceptible Echinochloa crus-galli plants^[1].