Pep4c

Pep4c is an inactive control peptide for Pep2m (HY-P1058). Pep4c lacks functional activity to disrupt Protein Interacting with C Kinase 1 (PICK1)-AMPA receptor (GluA2) or N-ethylmaleimide-sensitive factor (NSF)-GluA2 interactions. Pep4c is used as a negative control in experiments to validate the specificity of Pep2m's effects on AMPA receptor trafficking and synaptic plasticity^{[1][2][3][4][5][6]}. In Vitro:

Pep4c does not reduce the current response to topically applied AMPA, nor does it affect the frequency and amplitude of AMPA-mEPSCs in cultured hippocampal neurons^[1].

Pep4c does not alter the surface expression of AMPA receptors in cultured hippocampal neurons but improves the colocalization of GluR2 and synaptophysin in cultured hippocampal neurons^[1].

Pep4c does not affect long-term depression (LTD) and basal parallel fiber excitatory postsynaptic current (PF-EPSC) in hippocampal slices, but can induce long-term potentiation (LTP) by 1 Hz parallel fiber (PF) stimulation^[2][3].

Pep4c does not bind to NSF and does not alter autistic EPSCs evoked in Pep2m-loaded neurons^[4].

Pep4c does not alter the release of radiolabeled D-aspartate ([³H]D-ASP) in rat hippocampal synaptosomes, either in the absence (not shown) or in the presence of 50 μM (S)AMPA^[5].

Pep4c does not affect tritium release induced by 10 μM NMDA/1 μM Glycine (HY-Y0966), but caused changes in [³H]D-ASP release induced by 10 μM NMDA/1 μM Glycine and 10 μM NMDA/1 μM Glycine/50 μM (S)AMPA^[5].

Pep4c does not attenuate synaptic transmission^[6].