Leupeptin acid
CAS No. : 24125-28-8
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| Cat. No. : | HY-122476 |
| M.Wt: | 442.55 |
| Formula: | C20H38N6O5 |
| Purity: | >98 % |
| Solubility: |
Leupeptin acid is a peptide byproduct present in the fermentation product of Streptomyces roseus MA839-A1, and also an intermediate in leupeptin biosynthesis. Leupeptin acid serves as a substrate for leupeptin acid reductase to generate Leupeptin (HY-18234)[2].
In Vitro:Leupeptin acid is reduced to Leupeptin (HY-18234) by solubilized leupeptin acid reductase from Streptomyces roseus MA839-A1 in an ATP- and NADPH-dependent manner; the enzyme has Km values of 4.2 × 10-5 M for ATP and 1.3 × 10-6 M for NADPH, and is strongly suppressed by leupeptin and antipain rather than elastatinal[2].
Leupeptin acid is biosynthesized as an intermediate to form leupeptin by the combined leucine acyltransferase and leupeptin acid synthetase-reductase complex from Streptomyces roseus MA839-A1 using acetyl-CoA, L-leucine (HY-N0486) and L-arginine (HY-N0455) as substrates[2].
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