Podocarpusflavone A

Podocarpusflavone A is a biflavonoid present in the leaves of Podocarpus henkelii, with anti-tumor, topoisomerase I inhibitory, antibacterial and antifungal activities. Podocarpusflavone A exhibits antibacterial activity against Enterococcus faecalis and Pseudomonas aeruginosa. Podocarpusflavone A shows weak activity against fungal pathogens. Podocarpusflavone A targets topoisomerase I and induces cell cycle arrest and apoptosis in MCF-7 cells. Podocarpusflavone A can be used for studies on cancer, bacterial and fungal infections^[1][2]. In Vitro:Podocarpusflavone A (PFA) (Serial two-fold dilutions from an initial 1 mg/mL; 12-24 h) shows broad-spectrum bactericidal activity against Enterococcus faecalis and Pseudomonas aeruginosa (MIC = 60 μg/mL), with weaker activity against Staphylococcus aureus (MIC = 130 μg/mL) and Escherichia coli (MIC = 250 μg/mL)^[1].
Podocarpusflavone A (Serial two-fold dilutions from an initial 1 mg/mL; 24-48 h) exhibits weak antifungal activity against Candida albicans and Aspergillus fumigatus (MIC = 250 μg/mL) and Cryptococcus neoformans (MIC = 130 μg/mL) at 24 h and 48 h incubation^[1].
Podocarpusflavone A (CC₅₀ threshold of 1000 μg/mL; MIC values as measured in antibacterial and antifungal assays) has favorable selective antimicrobial activity (SI > 15) against Enterococcus faecalis and Pseudomonas aeruginosa, with lower selectivity against other tested bacterial and fungal pathogens^[1].
Podocarpusflavone A (PF) (1-40 µM; 72 h) shows potent antiproliferative activity against KB, MCF-7, DLD and HEp-2 tumor cell lines with ED₅₀ values of 4.67 µg/mL, 15.17 µg/mL, 4.95 µg/mL and 10.87 µg/mL^[2].

Podocarpusflavone A (20-40 µg/mL; 24 h) induces cell cycle arrest at sub-G1/S phase and triggers apoptosis in MCF-7 cells^[2].
Podocarpusflavone A shows moderate Topoisomerase I inhibitory activity with an IC₅₀ value of 65.98 µg/mL^[2].