DiFMUP
CAS No. : 214491-43-7
(Synonyms: 6,8-Difluoro-4-methylumbelliferyl phosphate)
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| Cat. No. : | HY-120166 |
| M.Wt: | 292.13 |
| Formula: | C10H7F2O6P |
| Purity: | >98 % |
| Solubility: | DMSO : 50 mg/mL (ultrasonic;warming;heat to 60°C) |
DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2]. In Vitro: DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].
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