Z-WEHD-FMK

Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity (IC₅₀=6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis^[1][2][4]. IC50 & Target:IC50: caspase-1/5; 6 μM (cathepsin B)^[1][2] In Vitro: Z-WEHD-FMK (80 μM; 9 hours) elicits a near-complete blockage of C. trachomatis-induced cleavage of golgin-84 and increases GM130 expression in cells^[1].
Z-WEHD-FMK (30?min before being exposed to?E. piscicida) effectively inhibits 0909I?E. piscicida?induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery^[2].
Z-WEHD-FMK (20 μM;18-24 hours following Cr³⁺,Ni²⁺, and Co²⁺) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr³⁺, it also induces a decrease of 35% to 45% with 48 ppm Ni²⁺ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co²⁺, down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co²⁺ in bone marrow-derived macrophages (BMDM)^[3].