2-Thiouridine

2-Thiouridine is an orally active modified nucleobase. 2-Thiouridine stabilizes U:A base pairs and destabilizes U:G wobble base pairs. 2-Thiouridine significantly improves the efficiency and accuracy of nonenzymatic replication of mixed-sequence A/U-containing RNA templates. 2-Thiouridine exhibits antiviral activity against multiple positive-sense single-stranded RNA viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ( [HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV)^[1][2][3][4]. In Vitro:2-Thiouridine (0.625-40 μM) inhibits the replication of ssRNA+ viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ([HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV) in a dose-dependent manner^[4]. In Vivo:2-Thiouridine (50-150 mg/kg; p.o.; twice daily) prolongs the survival rate and reduces the viral RNA load in serum, spleen and other tissues in DENV2-infected mice^[4].
2-Thiouridine (2-300 mg/kg; i.v. (2-20 mg/kg; once a day) or p.o. (300 mg/kg; twice a day); 5 days) improves the survival rate and reduces the lung viral titer and inflammatory factor levels in the SARS-CoV-2 infection mouse model^[4].