Fusicoccin

Fusicoccin (Fusicoccin A), a fungal pytotoxin, is a stabilizer of specific 14-3-3 protein-protein interactions. Fusicoccin sabilizes H⁺-ATPase/14-3-3 cmplex in pants, maintaining the enzyme in activated state. Fusicoccin also stabilizes 14-3-3 protein interactions with binding partners containing a C-terminal 14-3-3 recognition motif (a mode 3 motif), such as ERα, GPIbα, TASK3, CTFR, and p53. Fusicoccin induces apoptosis in cancer cells and has anticancer activity^[1][2][3][4]. In Vitro: Fusicoccin (Fusicoccin A) stabilizes a complex between 14-3-3 and the stress response regulator GCN1, inducing GCN1 turnover and neurite outgrowth (EC₅₀=29 mM)^[3].
Fusicoccin A activates the plasma membrane H⁺-ATPase by stabilizing its binding to 14-3-3 proteins, which results in water loss and the wilting of infected plants. Fusicoccin A decreases the proliferation and migration of human GBM cell lines in vitro, including several cell lines that exhibit varying degrees of resistance to pro-apoptotic stimuli. The IC₅₀ growth inhibitory concentration of fusicoccin A is 92 µM in the U373-MG cells and 83 µM in the Hs683 glioma cells^[4].