3-CPs

3-CPs is a monofunctional furanocoumarin and a photoprotective agent targeting Staphylococcus aureus DNA, possessesing anti-UVB lethal activity. 3-CPs competitively intercalates into DNA, forming exclusively 4',5'-furan-side mono-adducts upon UVB irradiation, and irreversibly inhibits the formation of cyclobutane pyrimidine dimers. 3-CPs prevents UVB-induced DNA damage by preferentially binding to strong (AT)_n sites within the DNA, without inducing lethal interstrand DNA cross-links; the limited number of mono-adducts it induces can be efficiently repaired by bacteria. 3-CPs holds potential for use in the development of photoprotective formulations for skin diseases, as well as in studies investigating bacterial DNA photodamage repair mechanisms and the optimization of photochemotherapy safety^[1]. In Vitro:3-CPs (0.02875–0.23 mM; 20 min dark incubation followed by cumulative UVB irradiation for up to 150 s) demonstrates a consistent photoprotective effect against UVB-induced bacterial death in Staphylococcus aureus ISP 255 strain, exhibiting no concentration-dependent differences^[1].