Methotrexate metabolite

Methotrexate metabolite (DAMPA) is an active metabolite of Methotrexate. Methotrexate is a Folic acid (HY-16637) antagonist, widely used as an immunosuppressant. Methotrexate metabolite is an antimalarial agent that inhibits parasite growth under physiological folic acid conditions, with IC₅₀ values of 446 nM and 812 nM against folic acid-sensitive strains and highly resistant strains, respectively. Methotrexate metabolite exhibits pharmacokinetic characteristics of rapid clearance and extensive metabolism, with a mean clearance rate of 1.9 l/kg/h and a mean terminal half-life of 51 minutes. Methotrexate metabolite is promising for research in the field of inflammation^[2][3]. In Vitro:Methotrexate metabolite (100 mM, 24 h) is not cytotoxic in the Molt-4 human leukemic cell line^[3]. In Vivo:Methotrexate metabolite (8.6-10.1 mg/kg, 200 mg/m², i.v., 15 minutes) has pharmacokinetic properties of rapid clearance and extensive metabolism in adult male Rhesus monkeys (Macaca mulatta) (8.0-15.9 kg), with an average clearance rate of 1.9 l/kg/h and an average terminal half-life of 51 minutes^[1].