WST-8
CAS No. : 193149-74-5
| Size | Price | Stock |
|---|---|---|
| 100mg | $85 | In-stock |
| 250mg | $113 | In-stock |
| 1g | $278 | In-stock |
| 5g | $974 | In-stock |
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| Cat. No. : | HY-D0831 |
| M.Wt: | 600.47 |
| Formula: | C20H13N6NaO11S2 |
| Purity: | >98 % |
| Solubility: | DMSO : 10 mg/mL (ultrasonic);H2O : 80 mg/mL (ultrasonic;warming;heat to 60°C) |
WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
In Vitro:1. Solution preparation
1.1 Preparation of stock solution
Solvent: H2O
Concentration: 50 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 5 mM with cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells are seeded in a 96-well plate at a concentration of 1.0-2.0 × 103 cells/well/100μL
2.2 Add 10 μL WST-8 at a final concentration of 5 mM.
2.3 Incubate cells at room temperature or 37°C for 1-4 h.
2.4 The absorbance at 450 nm was determined with a microplate reader.
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