X-Gluc (Dicyclohexylamine)


CAS No. : 18656-96-7

18656-96-7
Price and Availability of CAS No. : 18656-96-7
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50mg $180 In-stock
100mg $324 In-stock
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Cat. No. : HY-15935
M.Wt: 603.93
Formula: C26H36BrClN2O7
Purity: >98 %
Solubility: DMSO : ≥ 30 mg/mL
Introduction of 18656-96-7 :

X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1]. In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc Dicyclohexylamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.

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