ATP-Red 1 (solution)

ATP-Red 1 (solution) is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).
Solvent and concentration: DMSO: 1 mM In Vitro: 1. Solution preparation
1.1 Stock solution
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Disperse and suspend cells in 100 μL PBS in a 96-well plate at a density of 2×10⁵.
2.2 Add ATP-Red 1 at a final concentration of 5-10 μM.
2.3 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
2.4 Remove ATP-Red 1 working solution.
2.5 Analyze with a fluorescence microscope (Ex/Em = 510/590 nm).