LDS-751

Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751^[1][2][3]. In Vitro:Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.Preparation of LDS-751 working solution
1.1 Preparation of the stock solution:
Prepare LDS-751 stock solution with DMSO.
1.2 Preparation of LDS-751 working solution:
Dilute the stock solution in serum-free cell culture medium or PBS to obtain working solution.
Note: Please adjust the concentration of Lds-751 working solution according to the actual situation.
2. Cell staining
2.1 Cell preparation
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Fix cells with 3.7% formaldehyde for 10 minutes, discard the fixative and rinsed with PBS for three times, 5 minutes each time.
2.3 Permeate cells with 0.2% Triton X-100 for 5 minutes, wash three times with PBS, 5 minutes each time.
2.4 Add 1 mL of LDS-751 working solution, and then incubate at room temperature for 1-5 minutes.
2.5 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.6 Wash twice with PBS, 5 minutes each time.
2.7 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
3. Precautions
3.1 It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
3.2 Detect the fluorescence as soon as possible to avoid fluorescence quenching.
3.3 It is recommended to use AntiFade Mounting Medium (MCE Cat. No.: HY-K1042) to slow the fluorescence quenching.
3.4 Please adjust the concentration of LDS-751 working solution according to the actual situation.