QX77

QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells^[1]. QX77 can impede self-renewal and promote differentiation of ES cells^[2]. IC50 & Target: CMA^[1] In Vitro: QX77 (48 hours) upregulates Rab11 expression levels in Ctns^-/- MEFs^[1].
In Ctns^-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells^[1].
Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane^[1].
QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells^[1].
QX77 protects cystinotic cells from the increased susceptibility to tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression^[1].
QX77 (10 μM; 0, 3 or 6 days) activates CMA and increases LAMP2A expression in D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology^[2].