m-Carboxycinnamic acid bishydroxamide

m-Carboxycinnamic acid bishydroxamide (CBHA) is a histone deacetylase inhibitor. m-Carboxycinnamic acid bishydroxamide modulates histone acetylation sites, alters DNA methylation and epigenetic status, increases global histone acetylation, alleviates transcription repression, and facilitates chromatin remodelling. m-Carboxycinnamic acid bishydroxamide can be used for the research of cloned embryo development and epigenetic regulation^[1][2][3]. In Vitro:m-Carboxycinnamic acid bishydroxamide (20 μM; 6 h) treatment of reconstructed androgenetic embryos improves blastocyst formation rate, supports derivation of Ca²⁺ES cell lines with normal karyotype, pluripotent marker expression, and altered imprinted gene expression and DNA methylation patterns relative to zygote-derived R1 ES cells^[1].
m-Carboxycinnamic acid bishydroxamide (5-50 μM; 10 hr) treatment of hand-made cloned buffalo embryos significantly increases blastocyst rate to 63.77% and reduces apoptotic index to 4.36 with 10 μM, while 5 μM increases total cell number to 396.85, and 20 μM increases apoptosis^[2].
m-Carboxycinnamic acid bishydroxamide (5-50 μM; 10 hr) treatment of hand-made cloned buffalo embryos increases global H3K9ac levels to IVF blastocyst levels, while 10 μM specifically reduces global H3K27me3 levels (though not to IVF levels)^[2].
m-Carboxycinnamic acid bishydroxamide (10 μM; 10 hr) treatment of hand-made cloned buffalo embryos upregulates OCT-4, NANOG, and BCL-XL expression, downregulates BAX expression, and does not alter expression of p53, CASPASE3, DNMT1, DNMT3a, or HDAC1^[2].
m-Carboxycinnamic acid bishydroxamide (20 μM; 10 h) treatment of mouse 1-cell cumulus cell SCNT embryos accelerates and enhances histone acetylation at H3K9, H3K18, and H4K5 sites without altering DNA methylation levels^[3].
m-Carboxycinnamic acid bishydroxamide (20 μM; 10 h) treatment of mouse cumulus cell SCNT embryos significantly enhances outgrowth formation, proliferation, and Oct4-positive cell abundance in vitro^[3]. In Vivo:m-Carboxycinnamic acid bishydroxamide (CBHA) (20 μM; incubation; 6 hours) increases androgenetic embryo blastocyst rate to 18.1%, improves imprinted gene expression patterns in derived Ca^ES cells, and supports chimera formation at rates up to 2.6% with multi-tissue contribution^[1].
m-Carboxycinnamic acid bishydroxamide (CBHA) (1-20 μM; in vitro exposure; 10 h post-reconstruction) improves mouse SCNT embryo preimplantation and post-implantation development, increases full-term live offspring rate to 3.6-3.8%, and boosts NT-ES cell derivation efficiency to 59% by enhancing blastocyst quality, reducing apoptosis, and promoting outgrowth proliferation^[3].