Crosstide
CAS No. : 171783-05-4
| Size | Price | Stock |
|---|---|---|
| 1mg | $132 | In-stock |
| 5mg | $528 | In-stock |
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| Cat. No. : | HY-P0315 |
| M.Wt: | 1164.23 |
| Formula: | C48H77N17O17 |
| Purity: | >98 % |
| Solubility: | H2O : ≥ 50 mg/mL |
Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt. In Vitro: 16HBE14o? cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].
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