Ac-DEVD-AMC
CAS No. : 169332-61-0
| Size | Price | Stock |
|---|---|---|
| 1mg | $55 | In-stock |
| 5mg | $155 | In-stock |
| 10mg | $250 | In-stock |
| 25mg | $425 | In-stock |
| 50mg | $638 | In-stock |
| 100mg | $895 | In-stock |
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| Cat. No. : | HY-P1003 |
| M.Wt: | 675.64 |
| Formula: | C30H37N5O13 |
| Purity: | >98 % |
| Solubility: | H2O : < 0.1 mg/mL |
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm[1][2][3].
In Vitro:Ac-DEVD-AMC fluorescence labeling[2]
(1) Preparation of caspase buffer: 0.5% Igepal CA-630, 10 mM HEPES (pH 7.4), 2 mM EDTA, 0.5 mM phenylmethylsulphonyl fluoride (PMSF) and 5 µg/mL leupeptin.
(2) PC12 cells were treated with 50 µM 6-OHDA for 20 h.
(3) Collect cells by centrifugation at 250 g for 5 min, wash with PBS, and then centrifuge at 250 g for 5 min.
(4) Resuspension cells with caspase buffer at a ratio of 2 µL/105 cells and lyse at 4°C for 20 min.
(5) Collect the lysate by centrifugation at 7200 g, 4°C and 10 min.
(6) Incubate 20 µL lysate with 100 µM Ac-DEVD-AMC at 30°C for 10 min.
(7) Measure caspase activity using a fluorescence spectrophotometer at an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
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