2-Aminoacetamide hydrochloride

2-Aminoacetamide hydrochloride (Glycinamide hydrochloride) is a derivative of Glycine (HY-Y0966). 2-Aminoacetamide hydrochloride forms cell-absorbable nanocomplexes with proteins (such as bovine serum albumin) through strong electrostatic interactions, promoting cellular uptake of related proteins. 2-Aminoacetamide hydrochloride synergizes with BMP2 to upregulate the expression of osteogenic marker genes (such as Col1a1, Alp, Runx2) and proteins (such as COL1, BSP), enhancing collagen synthesis. 2-Aminoacetamide hydrochloride synergizes with BMP2 to promote osteoblast differentiation in vitro and bone regeneration in vivo^[1]. In Vitro:2-Aminoacetamide (1 mM; 4 days for osteogenesis markers) hydrochloride significantly enhances collagen synthesis, alkaline phosphatase (ALP) activity, and the expression of osteoblast differentiation marker genes (Col1a1, Alp, Osx, Runx2, Bsp, Ocn) in MC3T3-E1 cells under osteogenic medium (OSM) or with bone morphogenetic protein 2 (BMP2)^[1].
2-Aminoacetamide (3 mg/mL; 4 h) hydrochloride readily forms submicron-sized nanocomplexes with proteins (e.g., bovine serum albumin, BSA) through strong electrostatic interactions^[1].
2-Aminoacetamide (0.5-5 mM; 24-72 h) hydrochloride partially retards the proliferation of MC3T3-E1 cells and human BMSCs on day 3 but does not induce cytotoxicity in either cell line on day 1^[1]. In Vivo:2-Aminoacetamide (1 M; administered via collagen sponge implantation) hydrochloride synergistically enhances bone regeneration in a mouse calvarial defect model when combined with 0.25 μg BMP2, as evidenced by increased bone volume (BV), bone volume/tissue volume ratio (BV/TV), trabecular number (Tb.N), and mature bone formation compared with BMP2 alone or other combinations^[1].