MQAE

MQAE is a chloride ion (Cl^-) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl^- ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm)^[1][2]. In Vitro:General Protocol

1 Preparation of MQAE working solution
1.1 Preparation of the stock solution
Dissolve 1 mg MQAE in 0.3066 mL DMSO to obtain 10 mM of MQAE.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of MQAE working solution
Dilute the stock solution in Krebs-hepes buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl₂, 1 mM MgCl₂, 16 mM glucose, pH 7.4) to obtain 5-10 mM of MQAE working solution.
Note: Please adjust the concentration of MQAE working solution according to the actual situation.

2 Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of MQAE working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS.

3 Storage
-20°C, 1 year. Protect from light.
4 Precautions
4.1 It is recommended to store the stock solution at -20°C or -80°Caway from light and avoid repetitive freeze-thaw cycles.
4.2 Please adjust the concentration of MQAE working solution according to the actual situation.
4.3 This product is for R&D use only, not for drug, household, or other uses.
4.4 For your safety and health, please wear a lab coat and disposable gloves to operate.