CAS No. : 161180-11-6
(Synonyms: 7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate)
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| Cat. No. : | HY-116141 |
| M.Wt: | 448.59 |
| Formula: | C29H36O4 |
| Purity: | >98 % |
| Solubility: | 10 mM in DMSO |
7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3]. In Vitro: MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].
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