7-Hydroxycoumarinyl arachidonate


CAS No. : 161180-11-6

(Synonyms: 7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate)

161180-11-6
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Cat. No. : HY-116141
M.Wt: 448.59
Formula: C29H36O4
Purity: >98 %
Solubility: 10 mM in DMSO
Introduction of 161180-11-6 :

7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3]. In Vitro: MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].

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