MART-1 (27-35) (human)

MART-1 (27-35) (human) is the amino acid fragment spanning positions 27 to 35 of the MART-1 protein, and it represents an immunogenic epitope recognizable by HLA-A2-restricted melanoma-specific tumor-infiltrating lymphocytes (TILs). MART-1 (27-35) (human) can be used in studies related to melanoma^[1][2]. In Vitro:MART-1 (27-35) (human) (0.1-10 μM; 2 h at room temperature then 24 h at 4 °C) binds to purified human HLA-A2 molecules, and this binding is detectable via fluorescent signals^[1].
MART-1 (27-35) (human) (10 μM; overnight at 4 °C for initial complex formation) forms an HLA-A2 complex, which almost completely dissociates within 24 h at 37 °C^[1].
MART-1 (27-35) (human) (1 μg/mL; 1 h incubation with T2 cells, 4 h cytolytic assay) is specifically recognized by human MART-1 (27-35)-specific, HLA-A2-restricted TIL clones LT8, LT11 and LT12, and induces T2 target cells to release high levels of ⁵¹Cr^[1].
MART-1 (27-35) (human) (0.0001-10 μg/mL; 1 h) sensitizes HLA-A*0201⁺ T2 cells to lysis by CLW anti-MART-1 (27-35) CD8⁺ cytotoxic T lymphocytes (CTLs) in a dose-dependent manner^[2].