YO-PRO-1
CAS No. : 152068-09-2
| Size | Price | Stock |
|---|---|---|
| 100μg | $336 | Get quote |
| 500μg | $840 | Get quote |
| 1mg | $1350 | In-stock |
| 5 mg | Get quote | |
| 10 mg | Get quote | |
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| Cat. No. : | HY-D0918 |
| M.Wt: | 629.32 |
| Formula: | C24H29I2N3O |
| Purity: | >98 % |
| Solubility: | 10 mM in DMSO;10 mM in DMSO |
YO-PRO-1 is a non-cell-membrane-penetrating green fluorescent dye. YO-PRO-1 cannot penetrate normal cell membranes but can penetrate the membranes of apoptotic and necrotic cells. YO-PRO-1 has a high affinity for DNA. When it is not bound to DNA, it emits almost no fluorescence, and when it binds to DNA, it can emit green fluorescence (Ex/Em: 488/530±30 nM). YO-PRO-1 is often used together with Propidium Iodide (HY-D0815) to analyze and identify apoptotic and necrotic cells[1][2]. In Vitro:Simple steps for YO-PRO-1 staining: Prepare cell/tissue samples; Wash the samples after incubating with YO-PRO-1 for 30 minutes to 1 hour. It can be co-stained with Hoechst 33342 (HY-15559), Propidium Iodide (HY-D0815), etc.; Microscopic observation and detection: Image by excitation with a 488 nm laser line[1][2].
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