2-Methoxycinnamaldehyde

2-Methoxycinnamaldehyde (o-Methoxycinnamaldehyde) is a natural compound that can be isolated from Cinnamomum cassia. 2-Methoxycinnamaldehyde inhibits topoisomerase-I/II and NF-κB signaling pathway, causes mitochondrial dysfunction, induces lysosomal vesiculation, thereby leading to DNA damage and cell apoptosis. 2-Methoxycinnamaldehyde exhibits antitumor effects^[1][2][3]. In Vitro:2-Methoxycinnamaldehyde (10-160 μM, 48 h) promotes the lactate dehydrogenase (LDH) release in cell NCI-H520, exhibits cytotoxicity, and inhibits the proliferation of NCI-H520 with an IC₅₀ of 12.11 μM^[2].
2-Methoxycinnamaldehyde (10-40 μM, 48 h) decreases mitochondrial membrane potential, activates caspase-3/9, induces apoptosis in NCI-H520^[2].
2-Methoxycinnamaldehyde (0-50 μM, 48 h) modulates expressions of cell cycle regulatory proteins, inhibits TNF-α-induced MMP-9 expression, and inhibits the migration of human aortic smooth muscle cells (HASMCs)^[3].
In Vivo:2-Methoxycinnamaldehyde (5-20 mg/kg, intratumoral injection, once daily for 49 days) inhibits the tumor growth and induces cell apoptosis of tumor cells in mouse NCI-H520 xenograft models^[3].