4-Nitrophenyl palmitate
CAS No. : 1492-30-4
(Synonyms: 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp)
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| 25g | $165 | In-stock |
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| Cat. No. : | HY-W013168 |
| M.Wt: | 377.52 |
| Formula: | C22H35NO4 |
| Purity: | >98 % |
| Solubility: | Chloroform : ≥ 100 mg/mL;DMSO : 6.67 mg/mL (ultrasonic;warming;heat to 60°C) |
4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase[1][2]. In Vitro:4-Nitrophenyl palmitate serves as a substrate for measuring esterolytic activity of porcine pancreatic lipase, Staphylococcus aureus protease, and Burkholderia sp. lipase via colorimetric detection of released p-nitrophenol, with turbidity issues resolved by adding Triton X-100 to the reaction mixture[1].
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