HATU

HATU is a third-generation uronium salt peptide coupling reagent. HATU increases the rate of peptide coupling reactions, activates amino acids, promotes peptide bond formation in both solution-phase and solid-phase synthesis, and also facilitates peptide assembly, fragment coupling, and linear peptide cyclization. HATU can promote the N-acylation of chitosan to generate amide-linked cationic derivatives with a controllable degree of substitution. HATU is commonly used in amine acylation reactions^[1][2]. In Vitro:HATU (0.3-3 eq; 2 h) mediates efficient, controlled N-acylation of chitosan in DMSO, enabling targeted DS values from ~10% to ~86% for TAC and successful one-pot mixed N-acylation with aromatic carboxylic acids, with reaction efficiency optimized via Design of Experiments^[2].