Dinitolmide

Dinitolmide (Zoalene) is an orally active anticoccidial agent used as a fodder additive. Dinitolmide has a wide anticoccidial spectrum with no effect on host immunity. Dinitolmide mainly acts on the first generation merozoite stage of coccidia and also has an inhibitory effect on the sporulation process of oocysts. Dinitolmide has anti-Toxoplasma gondii effect in vitro with an EC₅₀ of 3.625 μg/mL. Dinitolmide can be used to inhibits infections caused by Eimeria tenella^[1][2]. In Vitro:Dinitolmide (10-700 μg/mL, 24 h-7 d) potently inhibit T. gondii growth with slight cytotoxicity against Vero cells^[2].
Dinitolmide (20 μg/mL, 24 h) significantly inhibits the viability, invasion and proliferation of T. gondii tachyzoites in Vero cells^[2].
Dinitolmide (10 μg/mL, 20 h) results in asynchronous development of the inner membrane complex (IMC) in the daughter cells and the disruption of the membrane of T. gondii in Vero cells^[2].
Dinitolmide (10 μg/mL, 15 h) up-regulates genes related to cell apoptosis and nitric-oxide synthase and down-regulates many Sag-related sequence (srs) genes in T. gondii tachyzoites in Vero cells^[2].
In Vivo:Dinitolmide (125 mg/kg feed, p.o., free access to food for 7 days) significantly inhibits the infection of E. tenella in chickens^[1].