Thunberginol C

Thunberginol C is an orally active, selective, and non-competitive inhibitor of AChE and BChE, with IC₅₀ values of 41.96 and 42.36 μM, respectively. Thunberginol C exerts cytoprotective, pro-collagen type I restorative, MMP-1 inhibitory, hyaluronic acid restorative, anti-photoaging effects in skin cells. Thunberginol C exerts neuroprotective, anxiolytic, TNF-α inhibitory, neuroinflammation inhibitory, and oxidative stress inhibitory effects. Thunberginol C can be used for the research of Alzheimer’s disease, UVB-induced skin photoaging, allergic reactions, oral bacterial infections, and stress-induced anxiety^[1][2][3][4]. In Vitro:Thunberginol C non-competitively inhibits acetylcholinesterase (AChE) with an IC₅₀ of 41.96 μM and a K_i of 45.6 μM via van der Waals interactions at the enzyme's peripheral anionic site^[1].
Thunberginol C non-competitively inhibits butyrylcholinesterase (BChE) with an IC₅₀ of 42.36 μM and a K_i of 49.2 μM via a hydrogen bond and hydrophobic interactions at the enzyme's peripheral anionic site^[1].
Thunberginol C (1 μM; 24 h) significantly increases cell viability, type I procollagen and hyaluronic acid production and inhibits MMP-1 production in UVB-irradiated Hs68 human foreskin fibroblast^[2].
Thunberginol C (10 ppm) inhibits the growth of Bacteroides melaninogenicus and Fusobacterium nucleatum oral bacteria, with an MIC of 10 ppm for both species^[3].
Thunberginol C inhibits antigen-induced contraction of tracheal chain isolated from sensitized guinea pig, while showing little inhibition for histamine-induced contraction^[3].
Thunberginol C (1-30 μM; 24 h) protects primary cortical neurons against Corticosterone (HY-B1618)-induced cell death^[4]. In Vivo:Thunberginol C (2-20 mg/kg; p.o.; daily; 14 days) dose-dependently attenuates chronic restraint stress-induced anxiety, neuroinflammation, and oxidative stress in male CD-1 mice^[4].