ML216

ML216 (CID-49852229) is a potent, selective and cell permeable inhibitor of the DNA unwinding activity of BLM helicase with IC₅₀s of 2.98 μM and 0.97 μM for BLM^full-length and BLM^636-1298, respectively. ML216 inhibits ssDNA-dependent ATPase activity of BLM with a K_i of 1.76 µM. Antitumor avtivity^[1][2]. IC50 & Target: IC50: 2.98 μM (BLM^full-length) and 0.97 μM (BLM^636-1298)^[1] In Vitro: ML216 (12.5-50 µM; 24-72 hours; PSNG5 and PSNG13cells) treatment inhibits the proliferation of PSNF5 cells in a concentration-dependent manner, but not of PSNG13 cells^[1].
ML216 treatment leads to a statistically significant increase in the frequency of sister chromatid exchanges (SCEs) in PSNF5 cells, but not in PSNG13 cells^[1].
ML216 increases the sensitivity of PSNF5 cells to aphidicolin but has no sensitizing effect on isogenic PSNG13 cells devoid of BLM^[1].
ML216 inhibits both the full length WRN (IC₅₀ of 5 μM) and a truncated WRN^500-946 (IC₅₀ of 12.6 μM), with the former being 2.5-fold more sensitive to inhibition. BLM is a little more sensitive than WRN to inhibition by ML216 (1.7-fold based on IC₅₀ values). Despite the detectable inhibition of WRN by ML216, this compound appears selective for BLM in human cells. ML216 inhibits proliferation of WRN⁺ and WRN⁻ cells equally well, and similarly sensitized both cell types to aphidicolin^[1]. In Vivo: Although ML216 inhibits unwinding by the sequence-related BLM and WRN helicases similarly in vitro, the apparent dependence on BLM for ML216 to exert its biological effects in human cells suggests BLM specificity for the drug’s mechanism of action in vivo. A co-crystal structure of BLM in complex with inhibitor would be informative. Cellular cues in vivo may induce a specific conformation of WRN that makes it resistant to ML216^[2].