(R)-Sulforaphane

(R)-Sulforaphane (L-Sulforaphane) is a orally active, potent inducer of the Keap1/Nrf2/ARE pathway, exhibiting antioxidant and anticancer activities. (R)-Sulforaphane primarily functions by upregulating phase II detoxifying enzymes in cells, aiding in the removal of carcinogens and combating oxidative stress. (R)-Sulforaphane is capable of modulating gene expression, influencing various signaling pathways, including Nrf2, NF-κB, and AP-1. (R)-Sulforaphane can be used in studies of tumor biology, antioxidant defense mechanisms, as well as inflammation and immune responses^{[1][2][3][4][5][6][7]}. In Vitro:(R)-Sulforaphane (10 μM, 24 hours) pre-treatment of H9c2 cells reduces the number of apoptotic cells, decreases the expression of pro-apoptotic proteins (Bax, caspase-3, cytochrome c), and counteracts the increase in mitochondrial membrane potential induced by Doxorubicin (HY-15142A) (1 μM, 2 hours)^[4].
(R)-Sulforaphane (10 μM, 2 or 24 hours) pre-treatment enhances the expression of HO-1 in H9c2 cells and reduces the levels of ROS within the mitochondria (measured using MitoSOX Red reagent) induced by Doxorubicin (1 μM, 2 or 24 hours)^[4].