YM-1 (tosylate)

YM-1 tosylate is a stable MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 tosylate induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins^[1][2].
In Vitro:YM-1 tosylate promotes Hsp70-dependent steps in nNOS maturation and partially blocks formation of the ATP-bound form^[1].
YM-1 (0-200 μM) tosylate activates the binding of Hsp70 to its unfolded substrate^[1].
YM-1 (0.001-1000 μM) tosylate converts Hsp70 to its tight-affinity conformation and shows binding efficacy to Hsp70 with an IC₅₀ value of 8.2 μM^[1].
YM-1 (0, 0.1, 0.5 and 1 μM; 24 hours) tosylate promotes nNOS ubiquitination^[1].
YM-1 (5 and 10 μM; 24 and 48 hours) tosylate induces cell death of HeLa cells and growth arrest of hTERT-RPE1 cells^[2].
YM-1 (10 μM; 48 hours) tosylate up-regulates p53 and p21 proteins and down-regulates FoxM1 and survivin^[2]. In Vivo:YM-1 (1 mM; oral administration, for 7 days) tosylate rescues polyQ toxicity in Drosophila by activating Hsp70^[1].