CU-CPT22

CU-CPT22 is a potent protein complex of toll-like receptor 1 and 2 (TLR1/2) inhibitor, and competes with the synthetic triacylated lipoprotein (Pam₃CSK₄) binding to TLR1/2 with a K_i of 0.41 µM. CU-CPT22 blocks Pam₃CSK₄-induced TLR1/2 activation with an IC₅₀ of 0.58 µM^[1]. IC50 & Target: Ki: 0.41 μM (TLR1/2)^[1] In Vitro: CU-CPT22 is a toll-like receptor 1 and 2 (TLR1/2) inhibitor with an IC₅₀ of 0.58±0.09 µM. It is demonstrated that CU-CPT22 is able to compete with Pam₃CSK₄ for binding to TLR1/2 with an inhibition constant (K_i) of 0.41±0.07 µM, which is consistent with its potency observed in the whole cell assay. Increasing the concentration of CU-CPT22 to 6 µM decreases the anisotropy to background levels. It is found that CU-CPT22 inhibits TLR1/2 signaling without affecting other TLRs, showing it is highly selective in intact cells. CU-CPT22 is found to have no significant cytotoxicity at various concentrations up to 100 µM in RAW 264.7 cells. The result demonstrates that CU-CPT22 can inhibit about 60% of TNF-αand 95% of IL-1β at 8 µM^[1].