Gentamicin (sulfate)

Gentamicin sulfate, an aminoglycoside antibiotic, inhibits the growth of both gram-positive and gram-negative bacteria and to inhibit several strains of mycoplasma in tissue culture. Gentamicin sulfate inhibits DNase I with an IC₅₀ of 0.57 mM^[1][2][3]. IC50 & Target:IC50: 0.57 mM (DNase I)^[1] In Vitro:Gentamicin sulfate is a more effective in vitro bacterial inhibitor than combined penicillin-streptomycin, is nontoxic to tissue culture monolayers, and does not inhibit virus replication^[2].
Gentamicin sulfate has been used with success as an additive in commercial mycology media to inhibit growth of bacteria and has been shown to be bactericidal for a wider range of organisms (Pseudomonas aeruginosa, Proteus sp., and Streptococcus faecalis) than penicillin and streptomycin^[2].
Gentamicin sulfate does not interfere with the production of cytopathic effect by certain echoviruses and polioviruses in tissue culture, is nontoxic to Rhesus monkey kidney, HeLa, and human amnion cells, and is stable at autoclave temperatures^[2].
In Vivo:Gentamicin (10 mg/kg; s.c.; every 12 h for a total of six doses) sulfate improves survival and alters the inflammatory profile in a murine model of depsis from P. aeruginosa^[3].