LIN28 inhibitor LI71

LIN28 inhibitor LI71 is a LIN28 inhibitor that effectively inhibits LIN28:let-7 binding (IC₅₀: 7 μM). LIN28 inhibitor LI71 can abolish LIN28-mediated oligouridylation of let-7 precursor (IC₅₀: 27 μM). LIN28 inhibitor LI71 has potential application value in LIN28-driven cancer research. LIN28 inhibitor LI71 inhibits the interaction of cold shock protein of Plasmodium falciparum (PfCoSP) with DNA and α/β tubulin and has an inhibitory effect on Plasmodium falciparum^[1][2]. IC50 & Target:IC50: 7 uM (LIN28)^[1] In Vitro:LIN28 inhibitor LI71 competitively blocks the interaction between LIN28 and let-7 precursor by directly binding to the cold shock domain (CSD) of LIN28 (IC₅₀: 7 μM)^[1].
LIN28 inhibitor LI71 (40 μM, 40 minutes) can effectively inhibit LIN28-mediated oligouridylation of let-7 precursor, thereby preventing the degradation of let-7 and restoring the level of mature let-7 (IC₅₀: 27 μM)^[1].
LIN28 inhibitor LI71 (100 μM) shows that it directly binds to CSD rather than zinc joint domain (ZKD) or let-7 RNA in saturation transfer difference (STD) spectroscopy experiments^[1]
LIN28 inhibitor LI71 (50-100 μM) can effectively inhibit cell viability in HeLa cells expressing LIN28A and LIN28B without significant cytotoxicity^[1].
LIN28 inhibitor LI71 (100 μM, 48 hours) significantly increases the expression level of mature let-7 in leukemia cells (K562) and embryonic stem cells (mESCs), thereby inhibiting cell viability^[1].
LIN28 inhibitor LI71 inhibits the interaction of PfCoSP with DNA and α/β tubulin by competitively binding to PfCoSP^[2].
LIN28 inhibitor LI71 (1.95-250 nM, 72 hours) has IC_50s of 1.35 nM and 6.3 nM in 3D7 and RKL-9 strains of Plasmodium falciparum, respectively^[2].
LIN28 inhibitor LI71 (10-500 nM, 30 minutes) affects the gametogenesis of Plasmodium falciparum and inhibits the deflagellation of male gametes in vitro^[2].