Alizarin Red S Indicator (4.3-6.3), IND

Alizarin red S indicator (C.I. 58005) is a reductively active (quinone-based) anthraquinone dye that forms complexes with metal ions (such as zirconium, calcium) or boric acid to label calcium deposition and perform electrochemical sensing functions. Alizarin Red S undergoes reversible redox reactions (for electrochemical detection) and irreversible chelation (for bone staining). Alizarin Red S is mainly used in bone metabolism research (labeling mineralized tissue), sugar detection (boric acid-sugar competition system) and metal ion sensing (such as zirconium ion detection)， and can be used in osteoporosis and metabolic disease research^[1][2][3]. In Vitro:Alizarin Red S (0.1 mM) is used as an electrochemical indicator in cyclic voltammetry. Through interaction with Phenylboronic Acid (HY-W001090) and the competitive effect of fructose, fructose is detected with a linear range of 50 mM^[1].
Alizarin Red S (0.05 M; 5 min) is immobilized on a triacetylcellulose membrane after forming an ion pair with hexadecyltrimethylammonium bromide (CTAB) to construct a zirconium ion optical sensor. The detection range of Zr[4+] is 0.39-301 μg/mL, and the detection limit is 0.08 μg/mL^[2]. In Vivo:Alizarin Red S (100 mg/kg, 125 mg/kg; ip; single dose) rapidly (2 min) labels all bone surfaces in 4-60 day old Wistar rats, with the width of the staining line reflecting bone formation and resorption activity, and staining penetrating the entire bone plate. In Wistar rats older than 60 days, only the bone surface is labeled, and the staining intensity decreases with age, suggesting that the degree of mineralization affects the resorption pattern. The injection solution is a 2% Alizarin Red S solution prepared in 0.45% sodium chloride (HY-Y0344H) ^[3].