Triisobutyl phosphate

Triisobutyl phosphate is an environmental pollutant that undergoes biodegradation. TiBP can be degraded in activated sludge via hydrolysis, dehydrogenation and hydroxylation, a process mediated by an acclimated microbial community with Pseudomonas as the key strain, and enhanced by TiBP-induced enzymatic activity. TiBP exerts toxic effects on zebrafish embryos^[1][2]. In Vitro:Triisobutyl phosphate (50 mg/L; 10 h) can be biodegraded by activated sludge acclimated through 71 cycles via hydrolysis, hydroxylation and dehydrogenation pathways, yielding 7 transformation products^[2]. In Vivo:Triisobutyl phosphate (50 mg/L initial concentration; static aquatic immersion; 24 hours) and its early biodegradation products exhibit toxic effects on zebrafish embryos including reduced survival, growth inhibition, increased heart rate, impaired swimming behavior, and thyroid endocrine disruption, while 24 hours of biodegradation significantly reduces these toxic effects^[2].