Ammonium chloride, for cell culture

Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor. In Vitro:Ammonium chloride (NH₄Cl), a lysosomotropic agent that raises intralysosomal pH, reduces the yield of reovirus during infection of mouse L cells^[2]. In Vivo:Ammonium chloride (0.28 M in drinking water) promotes the survival of myocardial cells in vivo by decreasing contractile dysfunction, cardiac hypertrophy, inflammation, apoptosis and autophagy^[1].