Isatoic anhydride
CAS No. : 118-48-9
| Size | Price | Stock |
|---|---|---|
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| 100g | $15 | In-stock |
| 500g | $27 | In-stock |
| 1000g | $53 | In-stock |
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| Cat. No. : | HY-76222 |
| M.Wt: | 163.13 |
| Formula: | C8H5NO3 |
| Purity: | >98 % |
| Solubility: | 10 mM in DMSO |
Isatoic anhydride is a single-adduct forming reagent and a monofunctional SHAPE reagent. Isatoic anhydride reacts with the 2'-hydroxyl group of RNA nucleotides via a single reactive group to form a single adduct, without forming cross-links between nucleotides. In SHAPE-JuMP experiments, Isatoic anhydride serves as a control reagent for single-adduct formation, which is used to distinguish true cross-links from single-adduct formation[1]. In Vitro:Isatoic anhydride (incubated for 15-30 min) forms monoadducts exclusively with the catalytic domain RNA of RNase P and VS ribozyme RNA[1].
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