9''-Methyl salvianolate B

9''-Methyl salvianolate B (MSB) is a blood-brain barrier-permeable inhibitor with high affinity for g5Rp, with a K_d value of 117 nM against African swine fever virus (ASFV) g5Rp. 9''-Methyl salvianolate B also acts as a ZBP1 inhibitor. It exhibits strong binding affinity to key proteins in the PPARγ/NF-κB pathway. 9''-Methyl salvianolate B blocks the interaction between ASFV g5Rp and host proteins eIF5A or RPS15. It restores hypusination modification of eIF5A, promotes autophagy (Autophagy), and inhibits ASFV replication. 9''-Methyl salvianolate B effectively disrupts ZBP1-mediated PANoptosome assembly. It effectively alleviates myocardial ischemia/reperfusion injury. 9''-Methyl salvianolate B can be used in studies related to African swine fever^[1][2][3]. In Vitro:9''-Methyl salvianolate B (0.156-10 μM) binds to purified ASFV g5Rp protein with high affinity, with a K_d value of 117 nM determined by SPR^[2].
9''-Methyl salvianolate B (10 μM) exhibits low cytotoxicity in 3D4/21 cells, with a CC₅₀ of 16.22 μM, and the cell survival rate exceeds 80% at 10 μM^[2]. In Vivo:9''-Methyl salvianolate B (5 mg/kg/day, 2 h before the onset of initial ischemia) alleviates myocardial ischemia/reperfusion injury in mice^[3].