X-Gluc (cyclohexanamine)
CAS No. : 114162-64-0
| Size | Price | Stock |
|---|---|---|
| 100mg | $53 | In-stock |
| 250mg | $106 | In-stock |
| 500mg | $211 | In-stock |
| 1g | $421 | In-stock |
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| 10 g | Get quote | |
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| Cat. No. : | HY-15935B |
| M.Wt: | 521.79 |
| Formula: | C20H26BrClN2O7 |
| Purity: | >98 % |
| Solubility: | DMSO : 100 mg/mL (ultrasonic) |
X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
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