N-Acetyl-DL-methionine

N-Acetyl-DL-methionine is an orally active methionine analog and a glutathione (GSH) precursor that can be metabolized into cysteine in vivo. N-Acetyl-DL-methionine increases plasma methionine concentration and the molar percentage of plasma lysine in castrated sheep, and can partially meet the methionine requirement of growing rats. N-Acetyl-DL-methionine is absorbable from the lower digestive tract of sheep and undergoes ruminal microbial degradation in vivo. N-Acetyl-DL-methionine alleviates Acetaminophen (HY-66005)-induced hepatic GSH depletion in mice, maintains hepatocyte integrity, and promotes de novo synthesis of hepatic GSH. N-Acetyl-DL-methionine can be used in studies related to acetaminophen-induced liver injury^[1][2]. In Vitro: N-Acetyl-DL-methionine (NAM) is resistant to degradation by rumen microorganisms in vitro^[1]. In Vivo: N-Acetyl-DL-methionine (NAM) (0.74 g; abomasal infusion; twice daily; for 4 consecutive days) significantly increases the molar percentage of plasma lysine in healthy castrated sheep at all sampling time points^[1].
N-Acetyl-DL-methionine delivered via single oral administration at 6.44 g shows a 15.7% recovery rate in the abomasal digesta of healthy castrated sheep. The recovery rate of dietary methionine is significantly lower than that of the control group, whereas no significant difference in total abomasal methionine equivalents is detected between the control group and the DL-homocysteine thiolactone-treated group^[1].
N-Acetyl-DL-methionine combined orally with 400 mg/kg acetaminophen (HY-66005) in one single dose can remarkably relieve hepatic GSH depletion and maintain hepatocyte integrity induced by acetaminophen^[2].