DAz-1


CAS No. : 1112977-84-0

1112977-84-0
Price and Availability of CAS No. : 1112977-84-0
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1mg $414 Get quote
5mg $1034 Get quote
10mg $1960 Get quote
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Cat. No. : HY-115402
M.Wt: 238.24
Formula: C10H14N4O3
Purity: >98 %
Solubility: 10 mM in DMSO
Introduction of 1112977-84-0 :

DAz-1 is a sulfonic acid probe for living cells, which can directly detect sulfonic acid-modified proteins in living cells and is cell-permeable[1]. In Vitro: DAz-1 sulfonic acid detection methods under different situations[1]
1. Prepare 10 μM protein, or 50 μg cell lysate, or 200 μg protein lysate.
2. React with 50 or 100 μM H2O2 at room temperature for 20 minutes; then add 1 mM DAz-1 or 5% (v/v) DMSO and react at room temperature for 15 minutes.
3. Quench the reaction with 5 mM DTT and remove excess reagent with gel filtration.
4. Use 4-12% SDS-PAGE to separate proteins, transfer to PVDF membrane, and detect by WB of HRP-Streptavidin.

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