| Size | Price | Stock |
|---|---|---|
| 1mg | $414 | Get quote |
| 5mg | $1034 | Get quote |
| 10mg | $1960 | Get quote |
| 50 mg | Get quote | |
| 100 mg | Get quote | |
| We match the lowest price on market. | ||
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| Cat. No. : | HY-115402 |
| M.Wt: | 238.24 |
| Formula: | C10H14N4O3 |
| Purity: | >98 % |
| Solubility: | 10 mM in DMSO |
DAz-1 is a sulfonic acid probe for living cells, which can directly detect sulfonic acid-modified proteins in living cells and is cell-permeable[1].
In Vitro: DAz-1 sulfonic acid detection methods under different situations[1]
1. Prepare 10 μM protein, or 50 μg cell lysate, or 200 μg protein lysate.
2. React with 50 or 100 μM H2O2 at room temperature for 20 minutes; then add 1 mM DAz-1 or 5% (v/v) DMSO and react at room temperature for 15 minutes.
3. Quench the reaction with 5 mM DTT and remove excess reagent with gel filtration.
4. Use 4-12% SDS-PAGE to separate proteins, transfer to PVDF membrane, and detect by WB of HRP-Streptavidin.
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