TSQ

TSQ is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λ_max ~470 nm). TSQ can combine with Zn²⁺ in the presence of Ca²⁺ and Mg²⁺ to produce blue fluorescence (Ex/Em = 334/495 nm)^[1]. In Vitro:1. Solution preparation^[4]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1-30 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-30 µM with DPBS or other buffers.
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are grown on glass coverslips coated with Collagen, rat tail (HY-NP010).
2.2 Rinsed three times with Dulbecco's phosphate buffered saline.
2.3 30 μM TSQ in DPBS, mix well and incubate at 37°C for 30 minutes.
2.4 After the stained cells are rinsed three times with DPBS.
2.5 Fluorescence microscopy detection (Ex/Em = 334/495 nm).