CAS No. : 108963-70-8
(Synonyms: Spectrozyme PCa; Chromozym Pca diacetate)
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| Cat. No. : | HY-P0021A |
| M.Wt: | 773.83 |
| Formula: | C35H51N9O11 |
| Purity: | >98 % |
| Solubility: | H2O : 2 mg/mL (ultrasonic);DMSO : 25 mg/mL (ultrasonic;adjust pH to 4 with CH3COOH) |
D-Lys(Z)-Pro-Arg-pNA diacetate is a chromogenic peptide substrate of activated protein C (APC)[1].
In Vitro:Cleavage of Chromogenic Substrates.[1]
APC derivatives (2 nM) and D-Lys(Z)-Pro-Arg-pNA (15-2000 µM) are mixed in 0.1 M NaCl, 0.02 M Tris-HCl (TBS, pH 7.5) containing 2.5 mM Ca2+, 0.1 mg/mL BSA, and 0.1% PEG 8000.
The rate of hydrolysis was measured at 405 nm at room temperature in a Vmax kinetic microplate reader.
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