I2959

I2959 is widely used as a photoinitiator for photochemical cross-linking of hydrogels, and the combination of I2959 and CMA hydrogel has been used as a bio-ink for biological 3D printing applications. In addition, I2959 has a high free radical generation efficiency and is cytotoxic to rapidly dividing cell lines^[1]. In Vitro:Method for encapsulation of Saos-2 cells in photochemically cross-linked CMA hydrogels^[1]:
1. Human osteosarcoma cell line (Saos-2 cells) are propagated in RPMI medium containing 15% FBS, 1% L-glutamine, and 1% penicillin/streptomycin.
2. Cells are suspended in neutralized CMA solution (containing I2959 at 0.02% or 0.1% concentration).
3. 45 µL of cell suspension is added to each well of a 96-well plate and incubated at 37°C for 30 min for gelation (5000 cells/gel).
4. Gels are exposed to UV light (365 nm) for two different times (1 min or 10 min) for photocross-linking. Hydrogels not exposed to UV light were used as controls for cell studies.
5. Culture the cell-encapsulated hydrogels in α-MEM medium containing 50 µg/mL ascorbic acid, 10 mM β-glycerophosphate, 10% FBS, and 1% penicillin/streptomycin for up to 14 days.