2-Methylnicotinic acid imidazolide
CAS No. : 1055970-47-2
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| 10g | $2525 | Get quote |
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| Cat. No. : | HY-123992 |
| M.Wt: | 187.20 |
| Formula: | C10H9N3O |
| Purity: | >98 % |
| Solubility: | DMSO : 50 mg/mL (ultrasonic) |
2-Methylnicotinic acid imidazolide, a RNA-SHAPE electrophile, is a general cell-permeable probe of RNA structure. 2-Methylnicotinic acid imidazolide can probe RNA structure in vivo and in live cells with high accuracy and single-nucleotide resolution[1]. In Vitro: Application of 100 mM 2-Methylnicotinic acid imidazolide (NAI) to murine embryonic stem cells (ESCs) resulted in 5S rRNA modification in as little as one minute with suitable signal-to-noise ratio. This signal begins to plateau by 15 minutes. 2-Methylnicotinic acid imidazolide also modifies 5S rRNA in cultured human cancer cells, Drosophila S2 cells, yeast, and E. coli. 2-Methylnicotinic acid imidazolide can read out alterations in the RNA tertiary structure as a result of sampling critical mature ribosome conformations.
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