ARN14494

ARN14494 is a potent and selective serine palmitoyltransferase (SPT) inhibitor, with an IC₅₀ of 27.3 nM. ARN14494 affects the CNS in terms of anti-inflammation and neuroprotection. ARN14494 protects neurons from β-amyloid 1-42-induced neurotoxicity through a variety of mechanisms, including anti-oxidation, anti-apoptosis, and anti-inflammation. ARN14494 can be used for Alzheimer’s disease research^[1].
In Vitro:ARN14494 (1-10 μM) inhibits SPT activity in mouse primary astrocytes in a concentration-dependent manner^[1].
ARN14494 (10 μM, 24 h) decreases ceramide and dihydroceramide levels in primary cortical astrocytes treated with Aβ1-42^[1].
ARN14494 (10 μM, 0-24 h) inhibits oligomeric Aβ-induced production of pro-inflammatory molecules including NO, TNF-α, IL1β, TGF1β, and pro-inflammatory enzymes INOS and COX-2 in cultures of primary astrocytes^[1].
ARN14494 reduces Aβ neurotoxicity and caspase-3 activation treated with astrocyte-conditioned medium in cultures of cortical neurons^[1].